--- Operating System, Graphics card ---
OS: Ubuntu Release 12.04 (precise) 64-bit, Kernel Linux 3.2.0-35-generic, GNOME 3.4.2
GFX: nVidia FXQuadro 4500 512mb

--- Blender version with error, and version that worked ---
ERROR: Blender 2.65a SVN53473
WORKED: any previous version

--- Short description of error ---
Can't render Targa or PNG file with alpha channel when the RGBA option is active in the Output properties.

--- Steps for others to reproduce the error (preferably based on attached .blend file) ---
Select Targa or PNG format with the RGBA option enabled and render the animation. And the same file can be rendered correctly (with the alpha channel) in previous version.